Abstract
A Cr:LiSrAlFl laser, pumped with a diffraction-limited laser diode and mode locked with a saturable Bragg reflector, produces 90-fs pulses at 860 nm with a cw power as high as 88 mW in two beams. It is shown that this recently developed, compact, solid-state laser can be used as an excitation source for two-photon laser scanning microscopy. Morphological and functional images of neocortical and cerebellar neurons were obtained with submicrometer three-dimensional resolution. Single dendritic spines could easily be resolved deep in scattering tissue.
© 1996 Optical Society of America
Full Article | PDF ArticleMore Like This
B. C. Collings, J. B. Stark, S. Tsuda, W. H. Knox, J. E. Cunningham, W. Y. Jan, R. Pathak, and K. Bergman
Opt. Lett. 21(15) 1171-1173 (1996)
Y. F. Chen, S. W. Tsai, Y. P. Lan, S. C. Wang, and K. F. Huang
Opt. Lett. 26(4) 199-201 (2001)
R. Fluck, G. Zhang, U. Keller, K. J. Weingarten, and M. Moser
Opt. Lett. 21(17) 1378-1380 (1996)