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Rapid determination of the three-dimensional orientation of single molecules

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Abstract

A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.

© 2001 Optical Society of America

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