Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging

Not Accessible

Your library or personal account may give you access

Abstract

We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40 µm×60 µm×3 µm. With the technique presented, the structure–mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.

© 2003 Optical Society of America

Full Article  |  PDF Article
More Like This
Three-dimensional particle tracking with subnanometer resolution using off-focus images

Zhipeng Zhang and Chia-Hsiang Menq
Appl. Opt. 47(13) 2361-2370 (2008)

Three-dimensional tracking of small spheres in focused laser beams: influence of the detection angular aperture

Alexander Rohrbach, Holger Kress, and Ernst H. K. Stelzer
Opt. Lett. 28(6) 411-413 (2003)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved