Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Simultaneous wide-field imaging and spectroscopy of localized fluorophores

Not Accessible

Your library or personal account may give you access

Abstract

A method that combines fluorescence imaging and spectroscopy of single molecules at room temperature is presented. This approach allows us to identify a number of imaged molecules unequivocally by simultaneously recording their fluorescence emission spectra. Furthermore, their spectral characteristics not only allow us to separate different fluorescent labels quantitatively and qualitatively but also provide information on the microenvironment of the molecules. This new method was successfully tested on a system of yellow-green and red fluorescent 20-nm latex beads, and its usefulness in studies of biological systems was illustrated for a preparation of combined binary ratio labeling–fluorescence in situ hybridization-stained mouse chromosomes.

© 2004 Optical Society of America

Full Article  |  PDF Article
More Like This
Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy

Jenifer F. Ojeda, Changan Xie, Yong-Qing Li, Fred E. Bertrand, John Wiley, and Thomas J. McConnell
Opt. Express 14(12) 5385-5393 (2006)

Integrated endoscopy system for simultaneous imaging and spectroscopy for early lung cancer detection

Haishan Zeng, Mirjan Petek, Marjeta Tercelj Zorman, Annette McWilliams, Branko Palcic, and Stephen Lam
Opt. Lett. 29(6) 587-589 (2004)

In vivo flow cytometer for real-time detection and quantification of circulating cells

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin
Opt. Lett. 29(1) 77-79 (2004)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved