Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Raman sorting and identification of single living micro-organisms with optical tweezers

Not Accessible

Your library or personal account may give you access

Abstract

We report on a novel technique for sorting and identification of single biological cells and food-borne bacteria based on laser tweezers and Raman spectroscopy (LTRS). With this technique, biological cells of different physiological states in a sample chamber were identified by their Raman spectral signatures and then they were selectively manipulated into a clean collection chamber with optical tweezers through a microchannel. As an example, we sorted the live and dead yeast cells into the collection chamber and validated this with a standard staining technique. We also demonstrated that bacteria existing in spoiled foods could be discriminated from a variety of food particles based on their characteristic Raman spectra and then isolated with laser manipulation. This label-free LTRS sorting technique may find broad applications in microbiology and rapid examination of food-borne diseases.

© 2005 Optical Society of America

Full Article  |  PDF Article
More Like This
Near-infrared Raman spectroscopy of single optically trapped biological cells

Changan Xie, Mumtaz A. Dinno, and Yong-qing Li
Opt. Lett. 27(4) 249-251 (2002)

Real-time Raman spectroscopy of optically trapped living cells and organelles

Changan Xie, Charles Goodman, Mumtaz A. Dinno, and Yong-Qing Li
Opt. Express 12(25) 6208-6214 (2004)

Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy

Jenifer F. Ojeda, Changan Xie, Yong-Qing Li, Fred E. Bertrand, John Wiley, and Thomas J. McConnell
Opt. Express 14(12) 5385-5393 (2006)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved