Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Not Accessible

Your library or personal account may give you access

Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche, et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.

© 2005 Optical Society of America

Full Article  |  PDF Article
More Like This
Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Benjamin Rappaz, Pierre Marquet, Etienne Cuche, Yves Emery, Christian Depeursinge, and Pierre J. Magistretti
Opt. Express 13(23) 9361-9373 (2005)

High-resolution quantitative phase-contrast microscopy by digital holography

Christopher J. Mann, Lingfeng Yu, Chun-Min Lo, and Myung K. Kim
Opt. Express 13(22) 8693-8698 (2005)

Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy

Christopher G. Rylander, Digant P. Davé, Taner Akkin, Thomas E. Milner, Kenneth R. Diller, and Ashley J. Welch
Opt. Lett. 29(13) 1509-1511 (2004)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved