Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Aberration-free optical refocusing in high numerical aperture microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70μm for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen.

© 2007 Optical Society of America

Full Article  |  PDF Article
More Like This
Generalised adaptive optics method for high-NA aberration-free refocusing in refractive-index-mismatched media

Jiahe Cui, Jacopo Antonello, Andrew R. Kirkpatrick, Patrick S. Salter, and Martin J. Booth
Opt. Express 30(7) 11809-11824 (2022)

Variable immersion microscopy with a high numerical aperture

Keita Ishida, Kanta Naruse, Yuta Mizouchi, Yoshihiro Ogawa, Michio Matsushita, Takeshi Shimi, Hiroshi Kimura, and Satoru Fujiyoshi
Opt. Lett. 46(4) 856-859 (2021)

Phase retrieval for high-numerical-aperture optical systems

Bridget M. Hanser, Mats G. L. Gustafsson, David A. Agard, and John W. Sedat
Opt. Lett. 28(10) 801-803 (2003)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved