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Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

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Abstract

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.

© 2007 Optical Society of America

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