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Femtosecond Kerr-gated wide-field fluorescence microscopy

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Abstract

We present a Kerr-gated microscope capable of collecting diffraction-limited 2D fluorescence images with sub-100fs time resolution. The concept is based on the insertion of a solid-state optical Kerr gate into a wide-field microscope. In addition to the considerably improved temporal resolution, the wide-field design allows for simultaneous tracking of several objects and ultrafast fluorescence lifetime imaging of doped and heterogeneous surfaces. The ultrafast fluorescence dynamics of gold nanoparticles is presented as an example of the capabilities of the instrument.

© 2008 Optical Society of America

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