Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Automatic three-dimensional tracking of particles with high-numerical-aperture digital lensless holographic microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We present an automatic procedure for 3D tracking of micrometer-sized particles with high-NA digital lensless holographic microscopy. The method uses a two-feature approach to search for the best focal planes and to distinguish particles from artifacts or other elements on the reconstructed stream of the holograms. A set of reconstructed images is axially projected onto a single image. From the projected image, the centers of mass of all the reconstructed elements are identified. Starting from the centers of mass, the morphology of the profile of the maximum intensity along the reconstruction direction allows for the distinguishing of particles from others elements. The method is tested with modeled holograms and applied to automatically track micrometer-sized bubbles in a sample of 4mm3 of soda.

©2012 Optical Society of America

Full Article  |  PDF Article
More Like This
Automatic method for focusing biological specimens in digital lensless holographic microscopy

Carlos A. Trujillo and Jorge Garcia-Sucerquia
Opt. Lett. 39(9) 2569-2572 (2014)

Color lensless digital holographic microscopy with micrometer resolution

Jorge Garcia-Sucerquia
Opt. Lett. 37(10) 1724-1726 (2012)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved