Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Dual-modal imaging with non-contact photoacoustic microscopy and fluorescence microscopy

Not Accessible

Your library or personal account may give you access

Abstract

Simultaneous imaging of complementary absorption and fluorescence contrasts with high spatial resolution is useful for biomedical studies. However, conventional dual-modal photoacoustic (PA) and fluorescence imaging systems require the use of acoustic coupling media due to the contact operation of PA imaging, which causes issues and complicates the procedure in certain applications such as cell imaging and ophthalmic imaging. We present a novel dual-modal imaging system which combines non-contact PA microscopy (PAM) based on PA remote sensing and fluorescence microscopy (FLM) into one platform. The system enables high lateral resolution of 2 and 2.7 µm for PAM and FLM modes, respectively. In vivo imaging of a zebrafish larva injected with a rhodamine B solution is demonstrated, with PAM visualizing the pigment and FLM revealing the injected rhodamine B.

© 2021 Optical Society of America

Full Article  |  PDF Article
More Like This
Photoacoustic-fluorescence microendoscopy in vivo

Wenzhao Yang, Jiasheng Zhou, Weihao Shao, Myeongsu Seong, Pengbo He, Zhanhong Ye, Zhendong Guo, Lili Jing, and Sung-Liang Chen
Opt. Lett. 46(10) 2340-2343 (2021)

Dual modality reflection mode optical coherence and photoacoustic microscopy using an akinetic sensor

Richard Haindl, Stefan Preisser, Marco Andreana, Wolfgang Rohringer, Caterina Sturtzel, Martin Distel, Zhe Chen, Elisabet Rank, Balthasar Fischer, Wolfgang Drexler, and Mengyang Liu
Opt. Lett. 42(21) 4319-4322 (2017)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved